Coding

Part:BBa_K4739007:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-10)


VgrG3-Parkin fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3328
    Illegal PstI site found at 2546
    Illegal PstI site found at 2905
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2546
    Illegal PstI site found at 2905
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3328
    Illegal PstI site found at 2546
    Illegal PstI site found at 2905
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3328
    Illegal PstI site found at 2546
    Illegal PstI site found at 2905
    Illegal NgoMIV site found at 2328
    Illegal NgoMIV site found at 3385
    Illegal AgeI site found at 2179
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.



Source

Sequence of VgrG3 gene is from V. cholerae genome, sequence of Parkin is built from its amino acid sequence.

References